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KMID : 0364819910290060387
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Korean Journal of Microbiology
1991 Volume.29 No. 6 p.387 ~ p.391
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Effects of Cultivation Conditions on the Growth and Polyamine Composition in Methylobacterium extorquens AM! Growing on Methanol
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Eom Chi-Yong
Park Ki-Jung
Kang Bin-Goo
Kim Young-MIn
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Abstract
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1
Methylobacterium extorquens AM I growing on methanol as a sole source of carbon and energy was found to grow most rapidly (t,=6 h) at 30C in a mineral medium (pH 7.0) containing 0.5% (v/v) methanol which was agitated at 200, rpm (optimal cultivation condition). Cells grown under the optimal cultivation condition contained more spermidine, but less putrescine, than the cells grown on 2.5%(v/v) (t,=8 h) or at 20C (4=8 h). Cells cultivated under the optimal condition was found to contain more spermidine than the cells grown at pH 6.0 (4=7 h) and pH 8.0 (4=7.3 h). The cells growing at the stationary phase under the optimal condition accumulated more spermine or putrescine than the cells growing at the same phase on 2.5% (v/v) methanol or at pH 6.0 and pH 8.0, respectively. M. extorquens AMI grown in a medium agitated at 100 rpm ((j=8.8 h) contained less spermidine and spenmine than the cells grown under the optimal cultivation condition.
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